rnascope in situ hybridization ish multiplex version  (Advanced Cell Diagnostics Inc)

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet:

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques:

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green) and P2X3R (blue) mRNA. Mouse and human DRG were costained for NF200 protein and DAPI (cyan), respectively. B) The percentage of CGRP-positive cells that co-expressed P2X3R was significantly increased in human DRG compared to mouse. C) The percentage of P2X3R-positive cells that co-expressed CGRP was significantly increased in human DRG compared to mouse. D) The percentage of P2X3R-CGRP co-expressing neurons was significantly increased in human DRG compared to mouse. This was due to a significant decrease in the percentage of neurons expressing P2X3R-alone, but not CGRP-alone, in human. E) Histogram showing the diameter of CGRP, P2X3R, and CGRP-P2X3R co-expressing neurons in mouse and human DRG. F) Distribution of each neuronal population in mouse and human DRG. G) The distribution of NF200, CGRP, and P2X3R populations in mouse DRG. Two way ANOVA with Bonferroni ****p<0.0001.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of human DRG labeled with RNAscope in situ hybridization for CGRP (green) and P2X3R (blue), SCN10A (red), and DAPI (blue). B) SCN10A was expressed in 71% of all sensory neurons in human DRG. C) Histogram showing the diameter of all SCN10A expressing neurons in human DRG. D) SCN10A was exclusively expressed in almost all CGRP and P2X3R-expressing neurons. E) Distribution of SCN10A neuronal populations in human DRG.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x, B) 40x, and C) 100x images of human DRG labeled with RNAscope in situ hybridization for CGRP (green) and P2X3R (blue), SCN9A (red), and DAPI (cyan). D) SCN9A was expressed in 96% of all sensory neurons in human DRG. E) Histogram showing the diameter distribution of all SCN9A expressing neurons in human DRG. F) SCN9A was expressed in virtually all neurons in human DRG. G) Distribution of SCN9A neuronal populations in human DRG.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and KCNS1 (red) mRNA. Mouse and human DRG are costained for NF200 protein and DAPI (cyan), respectively. B) Representative 40x and C) 100x images of KCNS1 label in human DRG. D) There was no significant difference in the expression of KCNS1 in mouse nor human DRG. E) KCNS1 was expressed in significantly fewer P2X3R expressing neurons. F) Size profile of mouse and G) human KCNS1-expressing DRG neurons. H) Distribution of KCNS1 neuronal subpopulations in mouse and human DRG. Unpaired t-test or two way ANOVA with Bonferroni *p<0.05.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x, B) 40x, and C) 100x images of human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and KCNB1 (red) mRNA with DAPI (cyan). D) KCNB1 mRNA was expressed in ∼85.4% of all human sensory neurons. E) Size profile of all human KCNB1-expressing DRG neurons. F) KCNB1 was expressed in almost CGRP and P2X3R-expressing neurons, and a large majority of the CGRP/P2X3R-negative neurons. G) Distribution of KCNB1 neuronal subpopulations in mouse and human DRG.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and HCN1 (red) mRNA. Mouse and human DRG were costained for NF200 protein and DAPI (cyan), respectively. B) Representative 40x and C) 100x images of HCN1 label in human DRG. D) HCN1 was expressed in significantly more human sensory neurons (94.4%) than mouse (70.9%). E) HCN1 was expressed in almost all CGRP and P2X3R expressing neurons in human, but not in mouse. F) Size profile of mouse and G) human HCN1-expressing DRG neurons. H) Distribution of HCN1 neuronal subpopulations in mouse and human DRG. Unpaired t-test or two way ANOVA with Bonferroni *p<0.05, ***p<0.001.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and HCN2 (red) mRNA. Mouse and human DRG were costained for NF200 protein and DAPI (cyan), respectively. B) Representative 40x and C) 100x images of HCN2 label in human DRG. D) HCN2 was expressed in significantly fewer human sensory neurons (44.0%) than mouse (71.5%). E) HCN2 was expressed in a mixed population of CGRP, P2X3R, and NF200 (or non-CGRP/P2X3R) neurons in mouse and human DRG. F) Size profile of mouse and G) human HCN2-expressing DRG neurons. H) Distribution of HCN2 neuronal subpopulations in mouse and human DRG. Unpaired t-test or two way ANOVA with Bonferroni **p<0.01, *p<0.05.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and CHRNA6 (red) mRNA. Mouse and human DRG were costained for NF200 protein and DAPI (cyan), respectively. B) Representative 40x and C) 100x images of CHRNA6 label in human DRG. D) There was no difference in the percentage of neurons expressing CHRNA6 in human (43.8%) or mouse (30.3%). E) CHRNA6 was expressed in significantly more CGRP-expressing neurons in human than mouse. F) Size profile of mouse and G) human CHRNA6-expressing DRG neurons. H) Distribution of CHRNA6 neuronal subpopulations in mouse and human DRG. Unpaired t-test or two way ANOVA with Bonferroni *p<0.05.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and CHRNA9 (red) mRNA. Mouse and human DRG were costained for NF200 protein and DAPI (cyan), respectively. B) Representative 40x and C) 100x images of CHRNA9 label in human DRG. D) CHRNA9 mRNA was not expressed in mouse DRG but was expressed in 25.6% of all human sensory neurons. E) CHRNA9 was expressed in CGRP and P2X3R-positive neurons in human DRG. F) Size profile of human CHRNA9-expressing DRG neurons. H) Distribution of CHRNA9 neuronal subpopulations in human DRG. Unpaired t-test *p<0.05.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and CHRNA10 (red) mRNA. Mouse and human DRG were costained for NF200 protein and DAPI (cyan), respectively. B) Representative 40x and C) 100x images of CHRNA10 label in human DRG. D) There was no difference in the percentage of neurons expressing CHRNA10 in human (3.68%) or mouse (1.65%). E) CHRNA10 was lowly expressed in a mixed population of CGRP and P2X3R-positive neurons. F) Size profile of mouse and G) human CHRNA10-expressing DRG neurons. H) Distribution of CHRNA10 neuronal subpopulations in mouse and human DRG. Unpaired t-test or two way ANOVA with Bonferroni.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and TRPV1 (red) mRNA. Mouse and human DRG were costained for NF200 protein and DAPI (cyan), respectively. B) Representative 40x and C) 100x images of TRPV1 label in human DRG. D) TRPV1 was expressed in significantly more neurons in human (74.7%) than mouse (32.4%) DRG. E) TRPV1 in almost all CGRP and P2X3R-expressing sensory neurons in human. F) Size profile of mouse and G) human TRPV1-expressing DRG neurons. H) Distribution of TRPV1 neuronal subpopulations in mouse and human DRG. Unpaired t-test or two way ANOVA with Bonferroni. ****p<0.0001, ***p<0.001, **p<0.01.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and TRPA1 (red) mRNA. Mouse and human DRG were costained for NF200 protein and DAPI (cyan), respectively. B) Representative 40x of TRPA1 label in human DRG. C) Distribution of TRPA1 neuronal subpopulations in mouse and human DRG. D) TRPA1 was expressed in significantly more sensory neurons in mouse (55.2%) than human (16.3%). E) TRPA1 was expressed in significantly more P2X3R and CGRP/P2X3R-negative neurons in mouse than human. G) Size profile of mouse and H) human TRPA1-expressing DRG neurons. Unpaired t-test or two way ANOVA with Bonferroni. ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and CD68 (red) mRNA. Mouse and human DRG were costained for NF200 protein and DAPI (cyan), respectively. B) Representative 40x and C) 100x images of CD68 label in human DRG. CD68 mRNA was not present in any sensory neurons but was present in other cells. CD68 mRNA was localized to the extra-neuronal space, particularly in the epineurium in mouse and satellite cells in human.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and CRLF2 (red) mRNA. Mouse and human DRG were costained for NF200 protein and DAPI (cyan), respectively. B) Representative 40x and C) 100x images of CRLF2 label in human DRG. D) CRLF2 was expressed in significantly more neurons in mouse (70.4%) than human (4.57%) DRG. E) CRLF2 was more abundant in CGRP, P2X3R, and NF200 (non-CGRP/P2X3R neurons) in mouse than human. F) Size profile of mouse and G) human CRLF2-expressing DRG neurons. H) Distribution of CRLF2 neuronal subpopulations in mouse and human DRG. Unpaired t-test or two way ANOVA with Bonferroni. ****p<0.0001.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing

Journal: bioRxiv

Article Title: Quantitative differences in neuronal subpopulations between mouse and human dorsal root ganglia demonstrated with RNAscope in situ hybridization

doi: 10.1101/2020.03.06.981597

Figure Lengend Snippet: A) Representative 20x images of mouse and human DRG labeled with RNAscope in situ hybridization for CGRP (green), P2X3R (blue), and HDAC6 (red) mRNA. Mouse and human DRG were costained for NF200 protein and DAPI (cyan), respectively. B) Representative 40x and C) 100x images of HDAC6 label in human DRG. The majority of HDAC6 mRNA was localized to the nucleus (white arrows) and was also present in satellite cells. D) HDAC6 was expressed in significantly more neurons in mouse (86.6%) than human (58.0%) DRG. E) HDAC6 was more abundant in NF200-positive neurons in mouse compared to CGRP/P2X3R-negative neurons in human. F) Size profile of mouse and G) human HDAC6-expressing DRG neurons. H) Distribution of HDAC6 neuronal subpopulations in mouse and human DRG. Unpaired t-test or two way ANOVA with Bonferroni. ***p<0.001, **p<0.01.

Article Snippet: RNAscope in situ hybridization ( ish ) multiplex version 1 was performed as instructed by Advanced Cell Diagnostics (ACD).

Techniques: Labeling, In Situ Hybridization, Expressing